Using the Pneumatic method to estimate embolism resistance in species with long vessels: A commentary on the article “A comparison of five methods to assess embolism resistance in trees”

Comparisons among methods are essential to validate plant traits measured across studies. However, a rigorous analysis is complex task that needs take into account not only the principle of method and its correct use, but also inherent intraspecific trait variability, something we feel fully considered by Sergent et al. (2020). They compared Bench dehydration, MicroCT, Pneumatic using three long-vesseled species found divergence these methods. As key finding, colleagues reported unreliable estimates ?50 for Olea europaea when in such species. Here, tested this finding measuring independently vulnerability curves O. europaea. Our results reinforce viability estimate embolism species, as already others. Briefly, discuss important procedures encourage further experiments, way know better limitations available improve our understanding about water relations.